Farantin Al'adu

Za'a iya raba farantin al'adun tantanin halitta zuwa kasa madaidaiciya da zagaye na kasa (mai siffa U da mai siffa ta V) gwargwadon fasalin kasa; yawan rijiyoyin gargajiya sune 6, 12, 24, 48, 96, 384, 1536, da sauransu; ya dogara da kayan, akwai farantin Terasaki da farantin al'ada ta al'ada. Zaɓin takamaiman ya dogara da nau'in ƙwayoyin halitta, ƙimar al'adun da ake buƙata da dalilai na gwaji daban-daban.

(1) Bambanci da zabi na faranti masu ala-fure da zagaye (nau'ikan U da siffofin V)

Hanyoyi daban-daban na faranti na al'ada suna da amfani daban-daban. Don ƙwayoyin halitta, ana yin amfani da ƙasa mai faɗi, wanda ya dace don kallo a ƙarƙashin madubin likita, yana da yanki a bayyane, kuma tsayin matakan ruwan kwayar yana da daidaito. Sabili da haka, yayin yin gwaje-gwaje irin su MTT, walau masu jituwa ko waɗanda aka dakatar da su, ana amfani da farantin ƙasa na ƙasa gabaɗaya. Dole ne a yi amfani da farantin al'adun ƙasa don auna ƙimar sha. Kula da kayan musamman. Lakabin "Al'adun Nama (TC) An Bi da shi" don al'ada ce. Ana amfani da faranti masu siffa-U ko masu siffar V gabaɗaya lokacin da ake buƙatar wasu buƙatu na musamman. Misali, a fannin rigakafi (immunology), lokacinda wasu nau'ikan lymphocytes daban daban suka cakuda al'adu, su biyun suna bukatar tuntubar juna don motsawa. A wannan yanayin, ana amfani da faranti masu fasalin U gabaɗaya saboda ƙwayoyin za su taru a cikin ƙaramin yanki saboda nauyi. Hakanan za'a yi amfani da farantin al'adun ƙasa don gwaje-gwajen haɗakar isotope, kuma ya zama dole ayi amfani da kwayar halitta don tattara ƙwayoyin halitta don al'adu, kamar "al'adun gargajiyar lymphocyte" da sauransu. Ana amfani da faranti masu kamannin V don kisan kwayar halitta da gwaje-gwajen hemagglutination. Hakanan za'a iya maye gurbin gwajin kwayar halitta da farantin U-mai siffar U (bayan ƙara ƙwayoyin, ƙara ƙarfin aiki a ƙananan gudu).

(2) Bambanci tsakanin farantin Terasaki da farantin al'ada ta al'ada

Terasaki farantin galibi ana amfani dashi don binciken kristallographic, kuma ƙirar samfurin ta dace da kallon kristal da nazarin tsarin. Akwai hanyoyi biyu na zama da mika digo, kuma bayyanar da tsarin kayan aikin aikace-aikacen hanyoyin guda biyu suma sun banbanta. Zaba polymer a matsayin polymer, kuma abu na musamman yana da kyau don lura da tsarin lu'ulu'u. Kwayar al'adar kwayar halitta galibi ana yin ta ne da kayan PS, kuma ana kula da abu mai wadatarwa, wanda ya dace da haɓakar salula da faɗaɗawa. Tabbas, akwai kayan haɓaka don ƙwayoyin planktonic, da kuma yanayin ƙasa mai ɗaurewa.

(3) Bambanci tsakanin farantin al'adun tantanin halitta da microplate

Faranti masu tambarin enzyme galibi sun fi farantin al'adun tantanin halitta tsada. Ana amfani da farantin salula galibi don al'adar kwayar halitta kuma ana iya amfani dasu don auna ƙimar furotin. Faranti da aka yiwa lakabi da enzyme sun hada da faranti masu rufi da faranti masu ɗaukar martani. Gabaɗaya, basa buƙatar amfani dasu don al'adun ƙwayoyin halitta. Ana amfani dasu galibi don aikin rigakafin garkuwar jiki. Gano furotin daga baya yana buƙatar buƙatu mafi girma da takamaiman ruwa mai lakafta enzyme.

(4) Yankin ƙasa na rijiyoyin faranti daban-daban na al'adu da adadin ruwan da aka ba da shawara kada ya yi zurfin gaske, gabaɗaya a cikin zangon 2 ~ 3mm, kuma ana iya amfani da yankin ƙasa na rijiyoyi daban-daban don yin lissafi kowane rijiya. Adadin ruwan da ya dace da za a saka (koma zuwa teburin da ke ƙasa). Idan adadin ruwan da aka kara yayi yawa, zai shafi musayar iskar gas (oxygen), kuma yana da saukin malalowa da haifar da gurbatawa yayin aikin motsawa. Specificayyadaddun ƙayyadaddun ƙwayoyin ƙwayoyin da za a ƙara ya dogara da manufar gwajin sassauƙa.